MicroRNA profiling of mammary gland and liver tissue of cows under different feeding conditions (control, restricted amount of milk replacer, milk replacer ad libitum). MicroRNA profiling of mammary gland and liver tissue of cows under different feeding conditions (control, restricted amount of milk replacer, milk replacer ad libitum)

To investigate the influence of the feed additive (milk replacer) on biological processes occuring in cow mammary parenchyma, mammary fat pad and liver via miRNA-mediated regulation. To this end, we performed microRNA expression profiling using next-generation sequencing. Overall design: Comparative gene expression analysis of miRNA-seq data for the investigated tissues and feeding conditions was performed.