MHC class I and MHC class II reporter mice enable analysis of immune oligodendroglia in mouse models of multiple sclerosis

Oligodendrocytes and their progenitors upregulate MHC pathways in response to inflammation, but the frequency of this phenotypic change is unknown and the features of these immune oligodendroglia are poorly defined. We generated MHC class I and II transgenic reporter mice to define their dynamics in response to inflammatory demyelination, providing a means to monitor MHC activation in diverse cell types in living mice and define their roles in aging, injury and disease. Reporter mice for MHC I (B2m-tdTomato) or MHC II (CD74-tdTomato) were immunized with MOG35-55 to induce EAE, then at peak EAE brain was dissociated at peak disease and sorted into tdTomato+ or tdTomato- then subjected to single cell RNA sequencing analysis