16S sequencing of C3H/HeJ mice

We performed 16S sequencing of fecal bacterial DNA from Tlr4Het and Tlr4C3H/HeJ mice pre and post- peanut sensitization to compare their gut commensal composition. Microbiome compositions were analogous betweenTlr4Het and Tlr4C3H/HeJ mice as shown by a comparison of the relative abundance of different bacterial classes found in the stool samples. This similarity is maintained even after peanut sensitization. Using both principle coordinate analyses (PCoA) and non-metric multidimensional scaling (nMDS) ordination, we further demonstrate that beta diversity of fecal bacteria is not different between Tlr4Het and Tlr4C3H/HeJ littermates. Lastly, Shannon indices revealed that alpha diversity of fecal bacteria is also similar in Tlr4Het and Tlr4C3H/HeJ littermates. Collectively, these data suggest that loss-of-function mutation in TLR4 did not lead to major alterations in gut microbiome of C3H/HeJ at steady-state or post sensitization.