Proteins identified by 2D-DIGE as the most differentially expressed in the ileum of mice two weeks post Echinostoma caproni infection.

TCA, tricarboxylic acid cycle; GABA, gamma-aminobutyric acid; Mit, mitochondrion; Cyt, cytoplasm; PM, plasma membrane; Nuc, nucleus; Px, peroxisome; ER, endoplasmic reticulum; Cell surf., cell surface; Mb raft, membrane raft; ECM, extracellular matrix; LC-MS/MS, liquid chromatography and tándem mass spectrometry; MS/MS, mass spectrometry.a The spot number corresponds to the number assessed to each spot in the 2D reference gel for DIGE data analysis and refers to the numbers in S2 Fig.b GI accession number in NCBI Protein databasec Average volume ratio quantified by DeCyder BVA moduledp value of Student’s t test calculated by DeCyder BVA modulee Subcellular localization according to UniProt databasef Percentage of amino acid sequence coverage for the identified proteins* The protein corresponding to spot number 2940 could not be properly identified by MASCOT 2.5, but it was successfully identified using ProteinPilot v4.5 search engine (Paragon algorithm) with the following parameters: trypsin specificity, iodoacetamide cysteine-alkilation, taxonomy restricted to Metazoa and the search effort set to rapid. Both coverage (%) and score values indicated for this protein are ProteinPilot values (a score > 2.0 means that it was identified with confidence ≥ 99%).Proteins identified by 2D-DIGE as the most differentially expressed in the ileum of mice two weeks post Echinostoma caproni infection.