Proline Glutamic acid and Leucine rich Protein 1 (PELP1) promotes glioblastoma progression by enhancing Wnt/ß-catenin signaling

We examined the transcriptional changes modulated by Proline Glutamic acid and Leucine rich Protein 1 (PELP1) by performing global transcriptome analysis. U87 cells were transfected with control siRNA or PELP1 siRNA and the RNA was isolated and utilized for RNA-seq analysis. Our results demonstrated that PELP1 modulated genes were related to inhibition of matrix metalloproteases, glioma invasion signaling, Wnt/ß-catenin signaling and other pathways related to invasion and GBM signaling. Overall design: Total RNA was isolated from U87 cells transfected with control siRNA or PELP1 siRNA. Illumina TruSeq RNA Sample Preparation was performed following manufacturer's protocol. Samples were run on an Illumina HiSeq 2000 in duplicate. The combined raw reads were aligned to UCSC hg19 and genes were annotated by Tophat. Genes were annotated and quantified by HTSeq-DESeq pipeline.