Association of Elevated Urinary miR-126, miR-155 and miR-29b with Diabetic Kidney Disease

TaqMan®Array Human MicroRNA Cards were used to profile the differential expression of human microRNAs in patients with CKD versus heathy controls TaqMan®Array Human MicroRNA Cards Array A v2.1 was used to profile the expression of 377 human microRNAs in pooled samples from 20 individual CKD patients and 20 healthy controls. This screening was used to identify new potential disease biomarkers for patients affected by CKD. Control pool (CP)1: urine samples from 5 females of average age 44.8 years; CP2: 5 females, 57.6; CP3: 5 males, 35.2; CP4, 5 males, 53.2. Patient Pool (PP)1: urine samples from 5 CKD3 patients with an eGFR between 43.3 and 36 mL/min per 1.73m2; PP2: 5 stage 3 patients, 35 - 31; PP3: 5 stage 4/5 patients, 27.3 - 23; PP4: 5 stage 4/5 patients, 22 - 12.