Targeting cell-matrix induced chemoresistance with regorafenib in a 3D model of osteosarcoma

Over the past four decades, there has been little advancement in treatment strategies for osteosarcoma (OS), the predominant primary bone tumor in the pediatric patient population. Current therapy involves multiple rounds of chemotherapy and surgical resection, which are associated with significant morbidity and suboptimal survival rates. A key challenge in developing new treatments is the difficulty in replicating the OS tumor microenvironment, particularly cell interactions with the extracellular matrix (ECM). This study uses an in vitro model of OS to investigate the cell response to collagen type I, the primary component of the osteosarcoma ECM. After seven days of culture within three-dimensional collagen hydrogels, OS cells displayed a more elongated cellular morphology and reduced sensitivity to the standard chemotherapy used for OS treatment compared to cells grown on two-dimensional substrates. To test whether this model could be used to study treatment strategies used for high..., , , # Targeting cell-matrix induced chemoresistance with regorafenib in a 3D model of osteosarcoma

Dataset DOI: [10.5061/dryad.w3r228128](10.5061/dryad.w3r228128)

## Description of the data and file structure

All raw data files are compressed into the .zip file - R_Rao_et_al_JBMR-A-25-0496.zip

Final Figures and Supplemental Data, and Tables are provided in the Zenodo Supplemental Information. 

Figure 1 - Osteosarcoma cell behavior across 2D and 3D culture. (b) These data depict the microarchitecture of collagen (COL) hydrogels in 2D and 3D configurations obtained through second harmonic generation imaging. (c) Quantification of mesh size from second harmonic generation images. (d) Cell viability images (green = live, red = dead) in the 2D tissue culture plastic (TCP), 2D COL, and 3D COL conditions. (e) Quantification of cell count through seven days of culture. (f) Cell morphology by F-actin staining (green) and nuclear (blue) visualization. (g) Quantification of cell density from the ...,