Figure_6.jpg

Immunofluorescence of NF-κB subunit p65 (green fluorescence – FITC) and actin filaments (F-actin, red fluorescence – ActinRed555) expressed in human dental pulp cells (HDPCs) after one and seven days of exposure to 0 (control) or 10 µg/mL LPS. The cell nuclei (blue fluorescence – Hoechst) were counterstained and the channels were merged (40×). After one and seven days, cell cultures without LPS (control) showed an expression of p65 mostly in the cytoplasm. Conversely, the exposure to 10 µg/mL LPS increased the nuclear translocation of p65 (pointers), demonstrating the activation of the NF-κB transcription factor. Chronic LPS exposure also abnormally affected the F-actin distribution around the nuclei after seven days (arrows).