Figure 1 + S3
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https://figshare.com/articles/dataset/Figure_1_S3/13440182
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资源简介：
At the top, the mutation strategy compared to the wild-type allele of
the TRIM5 gene. The mutation strategy consists in 8 introduced substitutions,
including 2 non-silent substitutions to mutate Arg332 into Gly (blue), 1
non-silent substitution to mutate Arg335 into Gly (blue), a silent mutation to
disrupt the PAM sequence (purple), a silent mutation to create a HaeIII
restriction site (green) and 3 silent mutations of the sequence targeted by
gRNA1 (grey). The HDR-modified region was amplified by PCR and the PCR products
were then analyzed by Illumina Miseq sequencing.
创建时间：
2020-12-20
