Jennifer Bestman, Holly Cline (2011) CIL:36844, Xenopus laevis, CNS neuron (sensu Vertebrata). CIL. Dataset

This is IMAGE #6 (50 minutes) of a time lapse series contained with in this image group. It shows neurons from the optic tectum of an albino Xenopus laevis tadpole CNS, stage 47.

The images were acquired with 10 minute intervals and show morphological plasticity of the dendritic arbor with branch additions and retractions.

These cells were transfected with in vivo electroporation of plasmid DNA encoding eYFP ~24 hours before the first image was acquired. The intact, living tadpole was anesthetized, positioned under a glass coverslip and this image was taken directly through its transparent skin using a custom built two-photon microscope.

Images were acquired every 10 minutes for 1 hour. After 60 minutes, the animal was placed in a 50 microMolar anisomycin solution for ~1.5 hours. A second time lapse series (10 minute interval for 60 minutes) was then acquired.

本描述呈现了图像组中包含的延时摄影系列的第6幅图像（拍摄时间为50分钟）。该图像展示了无色盲非洲爪蟾（Xenopus laevis）中枢神经系统视束中的神经元，处于发育的第47阶段。图像通过每隔10分钟获取一次，共持续1小时的方式获得，展示了树突树形态的可塑性变化，包括分支的增加与收缩。在首次图像采集前约24小时，通过体内电穿孔法将编码eYFP的质粒DNA转染至这些细胞中。实验中，完好的活体蝌蚪被麻醉，放置于玻璃盖玻片下，并通过定制构建的双光子显微镜直接透过其透明的皮肤进行拍摄。图像每隔10分钟采集一次，持续1小时。在60分钟后，将动物置于50微摩尔异亮氨酸溶液中处理约1.5小时。随后，再次进行了一个时长为60分钟、每隔10分钟的延时摄影系列采集。