Global analysis of gene expression in REF52 cells expressing SV40 T antigens

SV40 transforms cells through the action of two oncoproteins, large T antigen and small t antigen. Small t antigen targets phosphatase PP2A, while large T antigen stimulates cell proliferation and survival by action on multiple proteins, including the tumor suppressors Rb and p53. Large T antigen also binds components of the transcription initiation complex and several transcription factors. We examine global gene expression in SV40-transformed REF52 cells. SV40 transformation alters the expression of approximately 480 cellular genes. Much of this regulation is consistent with T antigen action on the Rb-E2F pathway.SV40（猴病毒40）通过两种癌蛋白——大T抗原（large T antigen）与小T抗原（small t antigen）的介导作用实现细胞转化。其中小T抗原靶向结合磷酸酶PP2A（phosphatase PP2A），而大T抗原则通过作用于包括肿瘤抑制因子Rb与p53在内的多种蛋白，促进细胞增殖与存活。此外，大T抗原还可结合转录起始复合物的组分以及多种转录因子。本研究对经SV40转化的REF52细胞开展全局基因表达分析，结果显示SV40转化可改变约480种细胞基因的表达水平，该调控效应的大部分与T抗原对Rb-E2F通路的作用机制相符。