Splicing events and transcriptome changes upon TDP-43 and FUS knock-down in human neuroblastoma cells

We performed exon array analysis to define and compare the transcriptomic changes, in terms of splicing and gene expression profilings, occurring in human neuroblastoma cells upon knocking down TDP-43 and FUS RNA-binding proteins (RBP), with the final aim to unravel the biological role of these RBPs in neuronal cells. To identify the differentially expressed and spliced genes, the microarray-based technology was performed using the recovered total RNAs from triplicate experimental knocked-down samples (siTDP-43 samples, siFUS samples and the control mock siCTRL samples).