IssA activity; MALDI-TOF MS
收藏DataCite Commons2025-04-01 更新2025-04-16 收录
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MALDI-TOF MS-based protease assays. Each reaction consists of 1mM substrate peptide and 1 micromolar IssA protease (IMG GeneID 2558297680; purified from heterologous Pichia pastoris). Reaction buffer was 10 mM sodium acetate, pH 5.2.
figure 5a = canonical flg22 as substrate (QRLSTGSRINSAKDDAAGLQIA)
figure 5b = Dyella japonica MF79 flg22 as substrate (ERLSSGMRINSAKDDAAGLAIS)
figure 5c = AtPEP1 from Arabidopsis thaliana as substrate (ATKVKAKQRGKEKVSSGRPGQHN)
figure 6s= canonical flg22 substrate as in figure 5a, but IssA was either inactivated by treatment with PMSF or mock treated.
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Mendeley Data创建时间：
2024-11-07
