Additional expression evidence for the predicted functional AS event.

aThe difference in cycle threshold (CT) value between the gene- or isoform-specific reaction and the non-template control reaction with the same oligonucleotides is given. Most of the “non-template” control reactions did not give any amplification after 40 cycles and in these cases a CT of 40 was taken for the calculation. Hence, the higher the number, the higher the expression level.
bNo oligonucleotides could be designed that are specific for the SVP1 splicing variant only. The indicated primers detect both SVP1 and SVP3.
The tissue for which the expression analysis was performed is indicated. The selection of tissue is based on a relative high expression of the fully spliced transcripts in the respective tissues. Isoform specific oligonucleotide sets were used (see Table S3).