beta 2 Adrenergic receptor expression analysis by flow cytometry on CD4+ T cells isolated from mPGES-1 KO or WT mouse splenocytes
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Flow cytometric data from five experiments analyzing beta-2-adrenergic receptor expression on CD4+ T cells (MACS positive selection from spleen) from mPGES-1 ko (-/-) or WT (+/+) mice subjected to vagus nerve stimulation (VNS) and intraperitoneal LPS injection 30 min prior to sample collection.<br><br>Panel (<i>more detailed info can be found in the "FCS file identification list" document</i>):<br>FITC-Viability<br>PE-b2AR<br>PCPCy5.5-CD69<br>PECy7-CD62L<br>APC-CD44<br>APCCy7-CD3<br>BV421-CD25<br>BV510-CD4<br><br>The protocol for staining, cell extraction VNS etc. is described in the publication "Microsomal prostaglandin E synthase-1 gene deletion impairs neuro-immune circuitry of the cholinergic anti-inflammatory pathway in endotoxaemic mpuse spleen" published in <b>PLOS ONE 2017</b> by the authors: Priya Revathikumar, Johanna Estelius, Utsa Karmakar, Erwan LeMaître, Marina Korotkova, Per-Johan Jakobsson and Jon Lampa. <br><br><br>



