Transcriptome and flower genes analysis of <i>Prunus campanulata</i> Maxim

<i>Prunus campanulata</i> Maxim. (syn. <i>Cerasus campanulata</i> or <i>Prunus cerasoides</i> var. <i>campanulata</i>) is a species of wild cherry native to Taiwan. Unlike most of the species in genus <i>Cerasus</i>, it is native to subtropical to tropical climate, and is often being planted as ornamental or roadside tree in city. Flower is the most attractive part of <i>P. campanulata</i>, but the genes participated in blossom in this specie are unknown. The transcriptome of <i>P. campanulata</i> inflorescence and leaves were sequenced by illumina technique. A total of 36,315 contigs were acquired, 22,531 of which were predicted as genes. For flower-related genes, 24 genes belong to MIKC group and one <i>FT</i>-like gene (<i>PcFTL</i>) belongs to PEBP gene family. Analysis of the <i>PcFTL</i> sequence suggests it as a flower activator. Expression of genes on the flower development pathway were also investigated, and the result showed genes belong to ABC model were highly expressed in inflorescence, thus indicates the <i>P. campanulata</i> transcriptome database is reliable. In this study an overview of <i>P. campanulata</i> inflorescence and leaves transcriptome was provided and the flower-related genes of <i>P. campanulata</i> were investigated. These findings improve our understanding of gene regulation of flowering in <i>P. campanulata</i>. <b>Abbreviation:</b> AGL - AGAMOUS LIKE; AP3: APETALA 3; BLAST - Basic Local Alignment Search Tool; CO - CONSTANTS; EMBL - European Molecular Biology Laboratory; EMF - Embryonic flower; FD - Flowering Locus D; FLC - FLOWERING LOCUS C; FPKM - fragments per kilobase of transcript per million; FRI - FRIGIDA; FT - Flowering Locus T; FTL - Flowering Locus T-like; gDNA - genomic DNA; GI - GIGANTEA; GO - gene ontology; LFY - LEAFY; MFT - MOTHER OF FT AND TFL1; NCBI - National Center for Biotechnology Information; NGS - next-generation sequencing; ORF - Open Reading Frame; PEBP - phosphatidylethanolaminebinding protein; PI - PISTILLATA; QTL - quantitative trait locus; SOC - SUPPRESSOR OF OVEREXPRESSTION OF CONSTANS 1; SSEP - SEPALLATA; SVP - SHORT VEGETATIVE PHASE; TAIR - The Arabidopsis Information Resource; TFL - TERMINAL FLOWER1-like