Identification of genome-wide binding sites of Psi, Myc and Pol in Drosophila imaginal wing discs using Targeted DamID

Targeted DamID was performed to identify binding targets of P-element somatic inhinitor (Psi), Myc and RNA Polymerase (using the RpII18 subunit common to all 3 polymerases). Scalloped(sd)-GAL4 was used to restrict expression to the wing disc. Dam methylase fusion constructs under the control of UAS were expressed for 24 hours using the scalloped-GAL4 driver in the background of temperature-sensitive GAL80. 3 replicates each of Psi-Dam, Myc-Dam and Pol-Dam were normalised to the corresponding Dam-only control.