Study of prevalence biofilm forming bacteria and broad-spectrum antibiofilm activity as anti-quorum sensing agent of streptomyces secondary metabolites on urinary catheter infections

Catheter-associated urinary tract infection is caused primarily by the multidrug-resistant biofilm forming bacteria of uropathogens. Theprevention and successful treatment of infections is difficult when catheters are colonized by bacterial biofilm growth. There have beenseveral efforts utilizing biocides in catheterizations, in attempts to inhibit all possible bacterial access to catheterized urethra. Nevertheless, itis clearly the case that such approaches are usually unsuccessful in the long run. Quorum quenching represents a new approach for control ofinfection for highly resistant microorganism. Therefore, there is a pressing need to find out natural Quorum Sensing inhibition compounds toovercome the resistance of the uropathogens. This unique concept of the antimicrobial control depends on reducing the burden of virulenceand suppress the development of antibiotic resistance rather than killing the bacteria. In the present investigation, the target microorganismswere isolated from attached surface of urinary catheters which evaluated quantitatively and qualitatively using standard techniques.Altogether 100 isolates were collected. Antibiotic susceptibility assays results showed that most of the isolates were resistant to a wide rangeof beta lactam and non-beta lactam antibiotics. Results of biofilm forming on microtiter plate and sterile foley catheter reveled that theseisolates possess a high capacity for biofilm formation on the both surface at the different degrees (strong, moderate and weak formingbiofilm) and required the only a short contact time for attachment. An anti-biofilm secondary metabolites from streptomyces strain sdLiexpressed broad-spectrum antibiofilm activity possibly by interfering with their QS systems against six different species producing strongbiofilm and multi-drug resistance. The anti-biofilm of secondary metabolites strain sdLi against six species was examined on sterilized coverglasses and urinary catheter sections using light microscope and scanning electron microscopy respectively. Evidence is presented that sdListreptomyces secondary metabolites showed broad-spectrum activity to attenuate the Quorum Sensing - dependent biofilm. Theinvestigation of quorum-sensing control may lead to the development of novel antipathogenic drugs.