Gene expression differences between leukemic cells from Mx1-Cre, Cbfb+/56M and Chd7f/f, Mx1-Cre, Cbfb+/56M mice by microarray

Results showed that Chd7 deficiency delay Cbfb-MYH11 induced leukemia, to explore the mechanism, We also performed microarray analysis on c-Kit+ leukemic cells to determine gene expression differences between Mx1-Cre, Cbfb+/56M and Chd7f/f, Mx1-Cre, Cbfb+/56M leukemic cells. Total RNA was extracted from c-Kit+ leukemia cells with the AllPrep DNA/RNA/Protein Mini Kit (QIAGEN). Three independent samples were obtained for each genotype. RNA quality was assessed using the Agilent BioAnalyzer 2100 (Agilent). Genome-wide RNA expression profiling was performed using Affymetrix GeneChip mouse Gene 2.0 ST Array (Affymetrix) according to the manufacturer’s instructions.