Total RNA sequence under control and PD-L1 knockdown

Detect overall RNA level change under PD-L1 knockdown. Overall design: The experiment is designed to determine the overall RNA level change between PD-L1 knockdown and control group. Briefly, we first infected MDA-MB-231 with either control shRNA lentivirus or PD-L1 knockdown shRNA lentivirus. After PD-L1 knowdown efficiency has been confirmed, RNAs from each group were extracted and sequenced. The downregulated genes for RNA seq were then defined by counts>100, folds>1.5 and p<0.02.