Gene expression data in murine nasal mucosa following nanoemulsion adjuvant exposure

Antigen uptake, processing, trafficking and presentation in nasal mucosal tissues are regulated by complex intra- and inter-cellular signalling events. Typical vaccine adjuvants lead to the transcription of pro-inflammatory cytokines and chemokines which relate to immune induction. We used microarrays to detail the global expression of genes in murine nasal mucosa underlying immune induction with a non-inflammatory nanoemulsion nasal adjuvant. 8 week old female CD-1 mice were nasally treated with 5 microliters/nare of either 20% (v/v) naomeulsion (W805EC) or PBS. Nasal epithelium was harvested immediately post-mortem at either 6 (6 hr) or 24 hours (24 hr) following treatment. The tissue was placed in OTC and frozen by immersion in liquid nitrogen. Total RNA was extracted per sample using RNA easy (Qiagen).