GRO-seq on MCF7 cells induced with sgRNA vectors targeting CTCF binding elements

We performed a CRISPR-based functional genetic screen targeting CTCF binding elements (CBEs) located in the vicinity of ERα-bound enhancers. The screen identified four functional CBEs whose targeting resulted in a marked negative effect on the proliferation of MCF-7 cells (ERα-positive cells) but no significant effect on MDA-MB-231 (ERα-negative cells) cells. Here, we carried out GRO-seq analysis on MCF-7 cells induced with an sgRNA vector targeting one of the hits detected by the screen: sgRNA_1118, as well as non-targeting sgRNA and sg1830 as control. GRO-seq in MCF7 cells induced with a sgRNA vector (sg1118) targeting a CTCF bidning element identified by a functional screen as well non-targeting sgRNA and sg1830 control vectors.