In-silico mutagenesis highlights key positions for Arg binding.

Energies are in kcal/mol. Calculated ΔG values of wild-type kinases are emphasized in bold and the rest of the energy values refers to the energy change associated with each mutation. Hyphen stand for cases in which no Arg binding position was detected at the investigated sub site. NC, no significant energy change (>±1 Kcal/mol) occurred upon mutation.