Expression data from cancer associated fibroblasts (CAFs) treated with OSM

The cytokine Oncostatin M (OSM) promotes cancer progression by acting as central node for multicellular interactions between cancer cells and surrounding stromal cells. OSM is mainly secreted by myeloid cells and the oncostatin M receptor (OSMR) is expressed by cancer cells and cancer associated fibroblasts (CAFs), among others. To understand the effect of OSM in CAFs, a small and well-annotated Clariom S gene microarray was performed in CAF-173 cells cultured in 3D spheroids and treated with OSM or vehicle (PBS). CAF-173 cells are inmortalised fibroblasts isolated from a human triple negative breast cancer tumour. CAF-173 cells were seeded in 3D spheroids and treated with 30 ng/ml OSM (or PBS) for 4 days before total RNA extraction in three independent experiments. RNA quality evaluation and microarray chips processing were performed at the Genomics Facility of the Biodonostia Health Research Institute.