OASA1 leaf senescence2

Cysteine plays a crucial role in various processes throughout plant growth and development stages. The gene OASA1 can produce cysteine in Arabidopsis. However, the potential developmental roles of OASA1 have not been explored during senescence. In the present study, the gene OASA1 showed increasing expression during senescence. Compared with Col-0, the mutant oasa1-1 and oasa1-2 showed late leaf senescence, which may be due to disturbed cysteine homeostasis. The mutant exhibited lower total cysteine content and reduced chlorophyll degradation. Meanwhile, WRKY75 promotes cysteine production by inducing the transcription of OASA1 expression, affecting leaf senescence. Our results demonstrate that the senescence-responsive transcription factor WRKY75 directly activates the expression of OASA1 to promote cysteine accumulation and H2O2 content, suggesting a mechanism by which senescence regulates cysteine accumulation in plants.