RNA-seq of Drosophila melanogaster with different genotypes after combinations of pro-longevity interventions

The search for new anti-aging interventions is an issue of great actuality. We report on the success of Drosophila melanogaster lifespan extension under the combined influence of dietary restriction, co-administration of berberine, fucoxanthin, and rapamycin, photodeprivation, and low-temperature conditions up to 185 days in w1118 strain and up to 213 days in long-lived E(z)/w mutants. The trade-off was found between longevity and locomotion. The transcriptome analysis showed an impact of pathways related to epigenetic regulation, lipid metabolism, cellular respiration, nutrient sensing, immune response, and autophagy in the registered effect.