Floral phenotype data from Mimulus parishii x M. cardinalis hybrids

Pollination syndromes are a key component of flowering plant diversification, prompting questions about the architecture of single traits and genetic coordination among traits. Here, we investigate the genetics of extreme floral divergence between naturally hybridizing monkeyflowers Mimulus parishii (self-pollinated) and M. cardinalis (hummingbird-pollinated).
We mapped quantitative trait loci (QTLs) for 18 pigment, pollinator reward/handling, and dimensional traits in parallel sets of F2 hybrids plus recombinant inbred lines and generated nearly isogenic lines (NILs) for two dimensional traits, pistil length, and corolla size.
Our multi-population approach revealed a highly polygenic basis (n = 190 QTLs total) for pollination syndrome divergence, capturing minor QTLs even for pigment traits with leading major loci. There was significant QTL overlap within pigment and dimensional categories. Nectar volume QTLs clustered with those for floral dimensions, suggesting a partially shared mod..., All hybrids in this study were generated from two highly inbred parental lines: Sierran CE10 for Mimulus cardinalis (Yuan et al., 2013) and PAR for M. parishii (Fishman et al., 2015; Nelson et al., 2021; Liang et al., 2023). F1 hybrids were generated with PAR as the seed parent and selfed to generate F2 seeds, while recombinant inbred lines (RILs) were generated by single-seed-descent from F2 individuals through 3-6 generations of self-fertilization (Sotola et al., 2023).
Plant growth conditions for QTL mapping - F2 hybrids were grown in two separate greenhouse common gardens at the University of Connecticut (UC) and the University of Montana (UM). At UC, seeds (CE10, PAR, F1 hybrids, F2 hybrids, and NILs) were sown into 98 cell trays (1.25\"sq x 2\"H), and then after four weeks, all seedlings were transferred to 18 pocket trays (3.25\" sq x 3.14\"H) and grown to flowering.  At UM, F2 seeds (and a few CE10 and PAR controls) were germinated on wet sand in Petri dishes, seedlings transplanted..., , # Floral phenotype data from Mimulus parishii x M. cardinalis hybrids

[https://doi.org/10.5061/dryad.6t1g1jx6r](https://doi.org/10.5061/dryad.6t1g1jx6r)

This dataset consists of floral phenotypes for three separate recombinant populations (UC_F2, UM_F2, RIL) used for QTL mapping of pollination syndrome divergence.  The first sheet defines the traits and the following sheets contain columns with the trait values measured for each individual (row) in each population. The corresponding genotypic data was previously archived. 

## Description of the data and file structure

Because some traits require additional handling (e.g., nectar volume, carotenoid values) and sometimes focal flowers are obviously deformed for one of the dimensional traits, the datasets are not perfectly complete for all individuals in each growout. Missing values are indicated by -.