Gene expression patterns of a Rhodopseudomonas palustris ΔregSR strain under photoheterotrophic growth conditions

The redox-sensing two-component signal transduction system, RegSR, in Rhodopseudomonas palustris has been shown to regulate an uptake hydrogenase in response to varying cellular redox states; however, its role is still largely undefined. Here, we used RNA sequencing to compare gene expression patterns in wild type R. palustris strain CGA010 to a ΔregSR derivative, CGA2023, under varying metabolic conditions. Growth conditions were chosen to utilize the different metabolic capabilites of R. palustris and, thus, present a variety of different redox challenges to the cell. Total RNA from two replicates of each strain was submitted for seven different growth conditions for a total of 28 samples. Cultures were provided with either high-light or low-light intensities in either nitrogen-fixing or non-nitrogen-fixing conditions. Carbon sources with varying redox states were provided including acetate, malate, and succinate as well as the aromatic compound benzoate.