Parameters for MM cell line electroporation.

Rule-of-thumb voltage settings for different HMCLs as deduced from electroporation with an expression plasmid for enhanced green fluorescent protein (pEGFP-N3). All transfections were carried out with a Gene Pulser (Bio-Rad) with a capacity setting of 960 µF. The whole unpurified cell culture was measured (FACS) at day one post-electroporation. The percentage ranges given are to be considered as guidelines for standard results based on between 10 (cell lines not regularly used in our experiments) and hundreds of electroporations (the regularly used “well-transfectable” MM lines). The best electroporations achieved have yielded up to 40% EGFP-positive cells, but suboptimal conditions - for example due to overly high cell densities in the preceding cell culture - may result in lower efficiencies than indicated.