Exploring the Effects and Mechanisms of Curcumin on Proliferation, Apoptosis, and Oxidative Stress in Hepatoblastoma cell line via the TLR4/TRAF6/MAPKs Pathway

A combination of bioinformatics and network pharmacology was used to screen curcumin's target genes and related signaling pathways in treating hepatocellular carcinoma. Curcumin-Containing Serum（CUR） was prepared. Cisplatin(CDDP) served as the positive control .The CCK-8 assay, scratch assay, and flow cytometry were used to assess curcumin's effects on HepG2 Cells cell proliferation, apoptosis, and cell cycle. ROS levels measurement, RT - qPCR, and Western blotting were conducted to explore curcumin's molecular mechanisms via TLR4/TRAF6/MAPKs signaling molecules.