Cattle Ileum RNA-Sequencing

Liver abscesses in cattle have a negative impact on cattle production and are a source of economic loss for producers across the beef cattle industry. Recently, a model has been developed to induce liver abscesses that will provide a method to better study and evaluate the mechanisms that underly their development. Using this model, calves were fed a diet to produce acidosis and these animals were also inoculated with bacteria typically isolated from liver abscesses to promote the development of liver abscesses. Salmonella has recently been isolated from liver abscesses, which has led to the theory that the intestinal tract may be contributing to liver abscess development via breakdown of the intestinal barrier known as leaky gut syndrome. In this study, seven of the 13 animals developed liver abscesses. Ileum tissue was collected from these animals and total RNA was isolated for RNA-sequencing.