Transcriptional profiling of Zygosaccharomyces bailii early response to acetic acid or copper stresses mediated by ZbHaa1

The non-conventional yeast species Zygosaccharomyces bailii is remarkably tolerant to acetic acid, a highly important microbial inhibitory compound in Food Industry and Biotechnology. ZbHaa1 is the functional homologue of S. cerevisiae Haa1 and a bifunctional transcription factor able to modulate Z. bailii adaptive response to both acetic acid and copper stresses. RNA-Seq was used to investigate genomic transcription changes in Z. bailii during early response to sublethal concentrations of acetic acid (140 mM, pH 4.0) or copper (0.08 mM), and uncover the regulatory network activated by these stresses under ZbHaa1 control. Overall design: mRNA profiling of Z. bailii IST302 parental strain (Wt) and derived deletion mutant Zbhaa1? (Mut) unstressed cells or exposed to either acetic acid (AA) or copper (Cu) stresses. A total of 18 samples were analysed (3 replicates per condition)