Serum starvation induces quiescence in ovarian cancer

While prior work in muscle cells indicates that metabolic reprogramming is associated with quiescence, whether metabolic changes occur in cancer to drive quiescence is unclear. We found that the metabolic enzyme ACSS2 is highly upregulated in quiescent ovarian cancer cells and overexpresssion of ACSS2 induces ovarian cancer quiescence. Overall design: We cultured Ovcar8 high grade serous cancer cells with or without 5% FBS for 48 hours. Controls were untreated. All samples were done in triplicate. Comparative gene expression profiling analysis for control and serum starved Ovcar8 cells.