Expression data from dissected caudal area of urogenital ridges of Sox11 knockout and wildtype E10.75 mouse embryos

The transcription factor Sox11 is a crucial regulator of kidney development. It is expressed in both mesenchymal and epithelial components of the early kidney anlagen. Deletion of Sox11 in mice causes an extension of the domain expressing Gdnf within rostral regions of the nephrogenic cord and results in duplex kidney formation. To gain insight into potential targets we performed microarray analysis on RNA isolated from the caudal region of E10.75 wildtype and mutant nephrogenic cords. 281 probe sets presented statistically significant changes (76 up, 205 down; FDR<0.1) Total RNA from dissected caudal area of urogenital ridges of Sox11 knockout and wildtype E10.75 embryos was extracted in 4 independent replicates for each genotype转录因子Sox11（transcription factor Sox11）是肾脏发育的关键调控因子。其在早期肾原基的间充质与上皮组分中均有表达。小鼠体内Sox11基因缺失会导致生肾索头侧区域的Gdnf表达域扩大，并引发重复肾形成。为探究其潜在靶基因，研究人员对分离自胚胎发育第10.75天（E10.75）野生型与突变型生肾索尾侧区域的RNA开展了微阵列分析（microarray analysis），共获得281个具有统计学显著差异的探针集（其中76个上调、205个下调，错误发现率（False Discovery Rate, FDR）<0.1）。此外，研究人员还从Sox11基因敲除型与野生型E10.75胚胎的尿生殖嵴解剖尾侧区域提取总RNA，每个基因型设置4次独立生物学重复。