Effect of cycloheximide in the co-translation mRNA degradation pattern in set2D and rrp6D strains

We investigated cryptic transcription start site usage, chromatin organization and post-transcriptional consequences in Saccharomyces cerevisiae. We used 5PSeq approach, which measures ribosome dynamics by sequencing the presence of co-translation mRNA degradation intermediates, to assess if cryptic transcripts are engaged in active translation. Here we assay the effect of Cycloheximide treatment (CHX) in the co-translational degradation profile of using strains where cryptic transcription is enhanced. We study the profiles in set2D and rrp6D, enhanced for the presence of chromatin-sensitive and RNA degradation-sensitive cryptic transcripts respectively. Overall design: We performed 2 biologically independent 5PSeq experiments. All cells were grown in YPD. CHX treated samples were incubated for 10 min and flash frozen in N2.