Effect of Pak4 deficiency on transcriptomic profile in eWAT of mice

The excessive energy is primarily stored as triacylglycerol in lipid droplets in adipocytes, which is hydrolyzed by the actions of lipases upon energy demand. In this study, the potential role of PAK4 in the lipolysis process in adipocytes was investigated. To elucidate functional outcomes, we performed RNA-sequencing analysis using littermate WT and adipose tissue-specific Pak4 KO mice in fasted conditions. Examination of RNA profile in eWAT of WT and adipose tissue-specific Pak4 KO mice in 24 h fasted condition过剩能量主要以三酰甘油的形式储存在脂肪细胞的脂滴中，当机体存在能量需求时，脂酶可将其水解。本研究探究了PAK4在脂肪细胞脂解过程中的潜在作用。为阐明相关功能效应，本研究以禁食状态下的同窝野生型（WT）小鼠与脂肪组织特异性Pak4敲除（KO）小鼠为模型，开展RNA测序（RNA-sequencing）分析，并对禁食24小时的上述两类小鼠的附睾白色脂肪组织（eWAT）中的RNA表达谱进行了检测。