Expression data from fasted macroH2A1/2 double knockout adult mouse liver

MacroH2As core histone variants have a unique structure that includes C-terminal nonhistone domain. MacroH2As are highly conserved in vertebrates, and are thought to regulate gene expression. However the nature of genes regulated by macroH2As and the biological significance of macroH2As for the organism remain unclear. Here we examine macroH2A function in vivo by knocking out both macroH2A1 and macroH2A2 in the mouse. We used microarrays to examine how the absence of macroH2A.1 and macroH2A.2 histone variants affect gene expression fasted adult mouse liver. Two month old male mice were fasted overnight (~16 hours). Mice were sacrificed between 9:00 and 10:00 AM, livers were collected and snap frozen with liquid nitrogen. Total RNA was extract with Trizol (life technologies) following standard protocol.MacroH2As核心组蛋白变体（core histone variants）具有包含C端非组蛋白结构域的独特结构。该类蛋白在脊椎动物中高度保守，被认为可调控基因表达。然而，其调控的基因本质以及该蛋白对生物体的生物学意义仍不明确。本研究通过敲除小鼠体内的macroH2A1与macroH2A2基因，在活体水平探究其功能。我们采用微阵列（microarray）技术，检测禁食成年小鼠肝脏中macroH2A.1与macroH2A.2组蛋白变体缺失对基因表达的影响。选取2月龄雄性小鼠，进行约16小时的隔夜禁食。于上午9:00至10:00之间处死小鼠，采集肝脏组织并使用液氮快速冷冻。按照标准实验流程，采用Trizol试剂（life technologies）提取总RNA。