Additional file 4: of Screening populations for copy number variation using genotyping-by-sequencing: a proof of concept using soybean fast neutron mutants

Copy number of templates amplified by 37 primer pairs in mutant and control lines as assessed by a ddPCR assay. ID: the identifier of the mutant; CNV_type: the type of CNV (homdel = homozygous deletion, hetdel = hemizygous deletion, dup = duplication); chr: the chromosome on which the CNV is located; start: the starting position of the CNV; end: the end position of the CNV; primer_pair: the identifier of the primer pair that was used for that assay; mutant_conc: the concentration (in number of DNA templates per μl) measured in the mutant for that assay; mutant_ref: the concentration (in number of DNA templates per μl) measured using reference primer pair BY in that mutant; mutant_n: the copy number of the templates amplified by primer_pair in the mutant as determined by dividing mutant_conc by mutant_ref; control_conc: the concentration (in number of DNA templates per μl) measured in the control for that assay; control_ref: the concentration (in number of DNA templates per μl) measured using reference primer pair BY in the control; control_n: the copy number of the templates amplified by primer_pair in the control as determined by dividing control_conc by control_ref; ratio: the ratio of the copy number of the mutant to the copy number of the control for the templates amplified by primer_pair. (CSV 4 kb)