Identifying and targeting angiogenesis-related microRNAs in ovarian cancer

The deregulation of microRNAs (miRNAs) has been demonstrated to be involved in tumor angiogenesis and offers opportunities for a new therapeutic approach. However, effective miRNA delivery systems are needed for such approaches to be successful. In this study, miRNA profiling of patient data sets, along with in vitro and in vivo experiments, revealed that miR-204-5p could promote angiogenesis in ovarian tumors through THBS1. To identify potential molecular mechanisms by which miR-204-5p exerts its pro-angiogenic effects, we performed a gene expression microarray of HeyA8-MDR cells following treatment with miR-204-5p-inh. Total RNA from control miR-inh- or miR-204-5p-inh-treated HeyA8-MDR cells was collected to assess global gene changes following miR-204-5p loss by gene expression assay. The array was analyzed using the Affymetrix Human Clariom S Assay.