Indo--1 Fluorescence and intracellular Ca2+ concentration in solution of Styl...

The fluorescence of the calcium dye Indo-1 was measured in solution by confocal microscopy at different calcium concentrations. A calibration curve was produced from the ratio...本研究采用共聚焦显微术（confocal microscopy），在不同钙浓度条件下对溶液中钙染料Indo-1的荧光信号进行了检测，并基于其荧光比值构建了校准曲线……