Transcriptome profiling of mRNAs in cortex of rats with cerebral ischemia and reperfusion
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https://www.ncbi.nlm.nih.gov/sra/SRP417162
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We analyzed transcriptomic characteristic of motor cortex from Sprague-Dawley rats brain with cerebral ischemia and reperfusion (CIR). Overall design: We selected 3 Sprague-Dawley rats with CIR. The motor cortex was stripped from brain tissue, and total RNAs were extracted according to the manufacturer's instructions. Qualified total RNA was further purified. We complete the construction of sequencing sample library. The number of fragments of each gene was counted, and then normalized. Finally, the FPKM value of each gene was calculated by Perl script.
创建时间:
2024-01-12



