To identify the dynamic changes of cytokines in the ApoE-/- mice atherosclerotic plaque, heart, and serum upon β-adrenergic stimulation (isoproterenol, 5 mg/kg body weight) at 0.5hrs, 1hrs, 3hrs and 2
Assays were performed as previously described (https://doi.org/10.1016/j.cels.2022.04.005). All steps were followed by three PBS washes. Cells were fixed in 4% paraformaldehyde (EMS Emgrid 15710) for
