Structural analysis of protein machineries using single particle cryo-EM

Structures of large macromolecular machineries were for long time not studied at atomic level as they were often too large for X-ray crystallography and as electron microscopy...长期以来，大型大分子机器的结构未能在原子尺度上得到研究，这是由于其往往体积过大，无法适配X射线晶体衍射（X-ray crystallography）技术，且电子显微镜（electron microscopy）……