Replication data for appendices in the doctoral thesis "Metabolic engineering of Komagataella phaffii for renewable methanol bioconversion into 3-hydroxypropionic acid"

This dataset contains the additional files associated with appendices I and III of the doctoral thesis "Metabolic engineering of Komagataella phaffii for renewable methanol bioconversion into 3-hydroxypropionic acid".

The first appendix (I) describes the construction of a Komagataella phaffii proof-of-concept strain for 3-HP production from renewable carbon sources by implementing the synthethic β-alanine pathway. The performance of this strain was evaluated in 24-deep-well plate cultivations using Buffered Minimal medium supplemented with either glycerol (BMG) or methanol (BMM) and compared to two K. phaffii strains expressing the malonyl-CoA pathway for 3-HP synthesis. The corresponding file (Additional file S1) contains the raw and processed data obtained from these cultivations.

The third appendix (III) details the experimental procedures and findings related to the study of the redox metabolism of two 3-HP-producing K. phaffii strains. The corresponding file (Additional file S3) contains raw and processed data from intracellular redox cofactor quantification, measured using a colorimetric kit. It also includes quantification of 3-HP and methanol in the supernatant samples.