Morphological and functional survival of mouse oocytes suspended in various dilution of EAFS10/10, cooled at indicated rates on Cryotops, and warmed at 117,500°C/min.

*“Morphological survival" means an oocyte exhibiting normal osmotic responsiveness during the removal of EAFS and exhibiting normal morphology after 2 hr incubation in M16. That is, in the column labeled “+ 2 hr in M16", after the vitrified oocytes were warmed and the EAFS was removed, the eggs were incubated in M16 medium for 2 hr before being assessed for morphological normalcy. These are the survivals shown graphically in Figures 1 and 2. In the column labeled “0 hr in M16", as soon as the EAFS was removed, the eggs were scored for morphological survival. This was immediately followed by partial dissection of the zonae, and the mixing of eggs and sperm for IVF. In nearly all cases, each treatment was repeated 6 to 9 times with about 6 oocytes per repeat. In column of % of oocytes develop to 2-cell, values with different superscripts were significantly different (P<0.05) by one-way ANOVA. Letters show the differences of survivals with the same warming rate, same diluted vitrification solution and various cooling rates, and there is no significant differences with same warming rate and same cooling rate and various diluted vitrification solutions.
†The number of oocytes developing to 2-cell embryos after IVF/the number recovered after vitrification.
‡Column 5/Column 4.