Sex differences in the developmental patterns of the cortical astroglial cell translatome. Sex differences in the developmental patterns of the cortical astroglial cell translatome

Employing translating ribosome affinity purification with RNA sequencing (TRAPseq) on cortical tissue to isolate astroglial-specific translatome from Aldh1l1-L10-GFP mice and examine sex (male + female) and age (postnatal days 1, 4, 7, 14, 35; adults (9 weeks)) dependent changes in the astroglial translatome. Overall design: Cortical lysate (N=53 independent samples) from male and female Aldh1l1-GFP reporter mice (C57BL/6 background strain) at 6 different postnatal time points was used for translating ribosome affinity purification (TRAP) to extract actively transcribed RNA and then used with Illumina Hi-Seq RNA sequencing. Four of the samples (P7 Female 10, P7 Female 6, P7 Male 2, P7 Male 6) were sequenced twice.