Sample Dataset for Single Cell Tracking

Here, we provide sample data to test scripts available at https://github.com/AltmeyerLab/ from pre-treatment imaging (“Live pre-treatment.zip”), post-treatment imaging (“Live post-treatment.zip”) and iterative staining (“Iterative staining.zip”). Additionally, there are .txt files, which were extracted from ScanR Analysis (analysis_parameters_live.txt and analysis_parameters_iterative_staining.txt) that can be used to test the matlab scripts. Finally, there is a Tracking_fiji.xls file, which is used to copy the values generated from the “Coord” table in the matlab script to import into the tracking plugin in FIJI, so that the tracks can be overlayed with the stack of the corresponding cells. Please refer to the guide ("Live+QIBC_guide.pdf") and the README file ("README_TestData.pdf") for detailed instructions and software dependencies.