The metabolome of an E. coli CRISPRi library identifies benefits of minimal metabolite levels and targets for engineering
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Grid employed for Docking grid1- bottom where argsuccinate bindsgrid2- up where arginine was bound MD simulations ran with Desmond. - see specifics in the publication 1516- AraTha 9GGJ chainAB1517- Ecoli_1TJ7_alternativedimer_wLig_EM1518- Ecoli_1TJ7_alternativedimer grid1 argininosuccinate1519- Ecoli_1TJ7_alternativedimer grid1 diaminopimelate1520- Ecoli_1TJ7_alternativedimer grid2 argininosuccinate1521- Ecoli_1TJ7_alternativedimer grid2 diaminopimelate1522- Ecoli_1TJ7_alternativedimer grid1 redocking cubic 10A3, 150k atoms, 5x200ns, 27-30Na+250ns/day
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2026-01-25
