Labcode 7- HIV neutralization Protocol PBMC Neutralization assay

This document describes the isolation of Peripheral Blood Mononuclear Cells (PBMCs) from buffycoat, the stimulation of PBMCs by PHA, and the determination of the neutralization sensitivity of HIV-1 strains using a PBMC neutralization assay. Two different protocols are in use for the PBMC neutralization assay, depending on whether viruses are tested for sensitivity to a polyclonal (serum) or monoclonal (antibody) neutralizing agent.