Gene expression profiles of primary myocardial cells treated with LPS and transfected siMETTL3 in rats

Methyltransferase-like 3 (METTL3) is the m6A methyltransferase which binds to METTL14 and wilms tumour 1-associated protein (WTAP) to form a complex catalyzing the m6A modification, the most abundant epitranscriptomic modification in eukaryotic mRNAs. And it has been demonstrated that m6A methylation mediated by METTL3 to be essential for inflammatory responses. To identify the messenger RNA (mRNA) profile in the primary myocardial cells that were treated with lipopolysaccharide (LPS) and/or small interference siMETTL3, six groups of primary myocardial cells were used for analysis.甲基转移酶样3（Methyltransferase-like 3，METTL3）是一类m6A甲基转移酶，可结合METTL14与肾母细胞瘤1相关蛋白（wilms tumour 1-associated protein，WTAP）形成复合物，催化真核信使RNA（messenger RNA，mRNA）中最丰富的表观转录组修饰——m6A修饰。已有研究证实，METTL3介导的m6A甲基化对炎症反应至关重要。为明确经脂多糖（lipopolysaccharide，LPS）和/或靶向METTL3的小干扰RNA（siMETTL3）处理的原代心肌细胞内的mRNA表达谱，本研究共设置6组原代心肌细胞用于分析。